BBa_K1500011

BBa_K1500011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1500011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kevin Yang

Date created: 2014-10-16 11:00:00

Date modified: 2015-05-08 01:10:46

IPTG-GFP-dam

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1500011_sequence (Version 1)

Description

Dam is involved in methylating DNA, overexpression causes 6.4 * 10^-4 /// 6.4 * 10^4 mutation rate increase, mostly catalyzing transversions. It requires no mutations and simple overexpression is sufficient to cause its mutagenic activity. In this plasmid, it is placed in the IPTG-GFP plasmid in order to characterize mutation rate and verify functionality of the dam mutator gene.

Notes

NA

Source

Dam was PCR'd from genomic DH5-alpha E. coli DNA and cloned into the IPTG-GFP plasmid backbone (K1500002).

Components

Access	Instance	Definition
public	BBa_K1500002	BBa_K1500002
public	BBa_B0032	BBa_B0032
public	BBa_K1500994	BBa_K1500994

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1500002	1,946	BBa_K1500002
BBa_B0032	955,967	BBa_B0032
BBa_K1500994	974,1810	BBa_K1500994

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1500011/1

Member of these Collections

• iGEM Parts Registry