BBa_K1510214

BBa_K1510214 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1510214
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kao Jung Chang
Date created: 2014-10-07 11:00:00
Date modified: 2016-02-12 09:00:45

Dispersin generator circuit



Types
DnaRegion

Roles
Conjugation

oriT

Sequences BBa_K1510214_sequence (Version 1)

Description

he Dispersin gene codes for an enzyme which catalyzes the hydrolysis of the extracellular matrix produced by Gram negative bacteria.With strong constitutive promoter,together with ribosomal binding site and signal sequence yebF, make dispersin work more accurately.

Notes

To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part.

Source

The promoter, J23100, comes from the 2014 distributed igem kit. The signal sequence, yebF, encoding signal protein exporting recombinant protein extracellular, is from E.coli K-12. By designing primer, we obtain it together with its naturally occurring ribosomal binding site using PCR and extraction.The dispersin is from Lyon INSA igem team. Finally, the terminator B0015 is from 2014 igem distribution.

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1510214/1