BBa_K1510915

BBa_K1510915 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1510915
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kao Jung Chang
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:10:48

ketoisocaproate decarboxylase (THI3) Circuit



Types
DnaRegion

Roles
oriT

Conjugation

Sequences BBa_K1510915_sequence (Version 1)

Description

This circui encodes a branched-chain amino acid transaminase derived from the THI3 gene from Saccharomyces cerevisiae. THI3 catalyzes the second step in isoamyl alcohol biosynthesis: conversion of α-ketoisocaproate to 3-methylbutanal via enzymatic reaction.

Notes

To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part.

Source

HI3 gene from Saccharomyces cerevisiae,BBa_B0034 is from 2014 igem distribution and BBa_B0015 is from 2014 igem distribution.

igem#sampleStatus
It's complicated
igem#status
Planning
 
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synbiohub#topLevel
BBa_K1510915/1