Types | DnaRegion
|
Roles | oriT
Conjugation
|
Sequences | BBa_K1510915_sequence (Version 1)
|
Description
This circui encodes a branched-chain amino acid transaminase derived from the THI3 gene from Saccharomyces cerevisiae. THI3 catalyzes the second step in isoamyl alcohol biosynthesis: conversion of α-ketoisocaproate to 3-methylbutanal via enzymatic reaction.
Notes
To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part.
Source
HI3 gene from Saccharomyces cerevisiae,BBa_B0034 is from 2014 igem distribution and BBa_B0015 is from 2014 igem distribution.