Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Raoul Martin, Drew Dunham
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:10:48
OsmY based secretion for purification
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1519123_sequence (Version 1) |
Description
By inserting the DNA sequence of a desired protein in our part, you will be able to secret your protein, bind it to a Nickel column, and cleave off the desired protein from the tag in very few steps. The OsmY-tag will allow you to have your protein fold in the periplam and form disulfide bonds. Once folded OsmY also allows the portein to be secreted into the media, which contains significantly less portion of endesired protein. The media containing the construct can there be run through a nickel column for further purification. Once elluted from the column, one can add TEV-His protease to cleave the tag off the desired protein. By re-running the protein in a nickel column, the desired portein will elute directly, while the tag and the TEV-His will stay bound to the column.Notes
None.Source
OsmY comes from the MG1655 E.coli strain.| Access | Instance | Definition |
| public public public public public public | BBa_I712074 BBa_B0034 BBa_K892008 BBa_K243004 BBa_K157011 BBa_J18918 | BBa_I712074 BBa_B0034 osmY ShortLinke His TEVsite |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I712074 BBa_B0034 BBa_K892008 BBa_K243004 BBa_K157011 BBa_J18918 | 1,46 55,66 73,678 687,698 707,724 733,753 | BBa_I712074 BBa_B0034 osmY ShortLinke His TEVsite |