BBa_K152011

BBa_K152011 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K152011
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Johnston Monje
Date created: 2008-10-30 12:00:00
Date modified: 2015-05-08 01:10:49

GFP RNAi construct



Types
DnaRegion

Roles
RNA

mature_transcript_region

Sequences BBa_K152011_sequence (Version 1)

Description

This part needs a strong promoter inserted to drive its expression. It could then be transfered into another plasmid by restriction with EcoRI and PstI. We intend to electroporate it into a corn plant endophyte Klebsiella pneumonii 342, and inject this into GFP Arabidopsis for testing of the bacteria's ability to silence plant genes using RNAi plasmids.

Notes

This part MAY have been made incorrectly (the antisense fragment got inserted in the sense orientation by accident). Stay tuned or try it out yourself to see what's up.

Source

Mix of two biobricks

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K152011/1