Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hector Sanguesa Ferrer, Max van
Date created: 2015-09-03 11:00:00
Date modified: 2016-01-25 11:29:45
pRha + CsgA + His-tag
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1583101_sequence (Version 1) |
Description
CsgA with HIS-tag attachted to the C-terminus under control of L-rhamnose-inducible promoterNotes
The nucleotides encoding for the second and third amino acid of the CsgA gene were changed to optimize synthesis success. (silent mutations)Source
The DNA was synthesized. The sequence of CsgA originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055). The design was based on the paper ""Programmable biofilm-based materials from engineered curli nanofibres""[1]1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.
| Access | Instance | Definition |
| public public public public public | BBa_G0002 BBa_K914003 BBa_K1583050 BBa_K1583008 BBa_K1583003 | SX scar BBa_K914003 BBa_K1583050 BBa_K1583008 BBa_K1583003 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_G0002 BBa_K914003 BBa_K1583050 BBa_K1583008 BBa_K1583003 | 1,8 9,130 131,155 156,167 169,660 | SX scar BBa_K914003 BBa_K1583050 BBa_K1583008 BBa_K1583003 |