Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stefan Robert Marsden
Date created: 2015-09-07 11:00:00
Date modified: 2016-01-25 11:31:05
pRha + CsgA with Hydroxyapatite-affinity tag & pTet + RFP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1583108_sequence (Version 1) |
Description
Upon induction with rhamnose, a fusion protein of CsgA with a hydroxyapatite affinity tag is expressed. The CsgA protein monomers can aggregate to form amyloid nanofibres. The affinity tag is supposed to increase adhesive properties of the biofilm created as a result of the CsgA induction.In order to better visualize the individual cells in the biofilm, we added the constitutive expression of RFP to our plasmid in a different operon. For our design, we took advantage of the double terminator present in the biobrick I13521 by placing it in front of our genes.
Notes
For our design, we took advantage of the double terminator present in the biobrick I13521 by placing it in front of our genes.Source
The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).| Access | Instance | Definition |
| public public | BBa_I13521 BBa_K1583102 | BBa_I13521 BBa_K1583102 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I13521 BBa_K1583102 | 1,923 932,1615 | BBa_I13521 BBa_K1583102 |