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BBa_K1583110

BBa_K1583110 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1583110

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Stefan Robert Marsden

Date created: 2015-09-03 11:00:00

Date modified: 2015-09-18 03:36:29

pRha + Mfp5_CsgA_His fusion protein & pTET + RFP

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Details

Types	DnaRegion
Roles	Coding CDS
Sequences	BBa_K1583110_sequence (Version 1)

Description

The fusion protein of Mfp5 with CsgA and N-terminal His tag was improved by insertion of constitutively expressed RFP in a different operon to make the cells red fluorescent.

Notes

The RFP operon was placed in front of the coding operon to take advantage of its double terminators.

Source

The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).

Components

Access	Instance	Definition
public public public public public public public public	BBa_I13521 BBa_K914003 BBa_K1583056 BBa_K1583058 BBa_K1583002 BBa_K1583012 BBa_K1583013 BBa_K1583059	BBa_I13521 BBa_K914003 BBa_K1583056 BBa_K1583058 BBa_K1583002 BBa_K1583012 BBa_K1583013 BBa_K1583059

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I13521 BBa_K914003 BBa_K1583056 BBa_K1583058 BBa_K1583002 BBa_K1583012 BBa_K1583013 BBa_K1583059	1,923 932,1053 1054,1080 1081,1092 1093,1323 1324,1353 1354,1746 1747,1767	BBa_I13521 BBa_K914003 BBa_K1583056 BBa_K1583058 BBa_K1583002 BBa_K1583012 BBa_K1583013 BBa_K1583059