Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Osthege, Sarah Lubjuhn, Laura Helleckes
Date created: 2015-09-05 11:00:00
Date modified: 2015-09-18 06:06:01
glgP targeting plasmid
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1585351_sequence (Version 1) |
Description
This plasmid is targeting glgP in the E. coli genome by expressing two sgRNAs. The complete glgP coding sequence is cut out and a stop codon is introduced by the repair templates.Notes
It is not designed to be BioBrick-compatible, but it can be used AS IS to knock out glgX in E. coli.It has to be used together with pCas (https://www.addgene.org/62225/)
Source
The part consists of genomic repair templates with a gene-edit and a synthetic sgRNA sequence.| Sequence Annotation | Location | Component / Role(s) |
| spy-tem cas9BIRE N20 for glgP targeting left pJ23119 pJ23119 N20 for glgP targeting right cas9BIRE spy-tem left homology arm right homolgy arm iGEM AACHEN MULTI STOP end of glgP cds glgA cds stop stop stop | 30,69 70,111 112,131 138,166 237,265 272,291 292,333 334,373 399,798 832,1231 799,831 721,798 851,1231 803,805 817,819 828,830 | feature/misc sequence_feature feature/misc sequence_feature feature/binding non_covalent_binding_site feature/promoter promoter feature/promoter promoter non_covalent_binding_site feature/binding sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/dna sequence_feature feature/dna feature/cds CDS feature/cds CDS feature/cds CDS stop_codon feature/stop feature/stop stop_codon feature/stop stop_codon |