BBa_K1595023

BBa_K1595023 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1595023
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonlin Chen
Date created: 2015-09-12 11:00:00
Date modified: 2015-09-18 01:38:54

MBP with EcnB from Escherichia coli str. K-12 substr. DH10B



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1595023_sequence (Version 1)

Description

Coding device for anti-microbial peptide derived from Escherichia coli EcnB with a stabilizing maltose-binding protein and TEV cutsite.

Notes

Use in conjunction with strain-expressing T7 polymerase.

Source

Synthesized by DNA 2.0

Sequence Annotation Location Component / Role(s)
Terminator
6xHist tag
EcnB
TEV cutsite
MBP
RBS
T7
1380,1418
1359,1376
1215,1358
1194,1214
81,1193
22,80
2,20
feature/misc sequence_feature
tag feature/tag
feature/cds CDS
feature/misc sequence_feature
CDS feature/protein
ribosome_entry_site feature/rbs
promoter feature/promoter
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1595023/1