Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonlin Chen
Date created: 2015-09-12 11:00:00
Date modified: 2015-09-18 01:47:12
EDA linked to cel5a via GSG linker
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1595030_sequence (Version 1) |
Description
Coding device for KDPG aldolase (EDA), a peptide-stabilizing protein, with a GSG linker and protein cel5a.Notes
Use in conjunction with strain-expressing T7 polymerase.cel5a is a stable, detectable protein that can be used to determine expression of our plasmid.
Source
Synthesize by DNA 2.0| Sequence Annotation | Location | Component / Role(s) |
| Terminator 6xHist tag BamHI cutsite Cel5a NdeI cutsite GSG linker EDA RBS T7 | 1989,2031 1971,1988 1965,1970 732,1964 729,734 720,728 81,719 22,80 1,21 | sequence_feature feature/misc tag feature/tag sequence_feature feature/misc CDS feature/protein feature/misc sequence_feature CDS feature/cds CDS feature/protein feature/rbs ribosome_entry_site promoter feature/promoter |