Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonlin Chen
Date created: 2015-09-17 11:00:00
Date modified: 2016-02-03 10:54:52
EcnA and EcnB from Escherichia coli str. K-12 substr. DH10B
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1595031_sequence (Version 1) |
Description
T7 + RBS + EDA + GSG + NdeI + EcnA + TEV + EcnB + 6xHist tag + BamHI + Terminator. Coding device for stabilizing protein EDA linked to EcnA/EcnB through a GSG linker.Notes
Use in conjunction with strain-expressing T7 polymerase.Source
Synthesized by DNA 2.0| Sequence Annotation | Location | Component / Role(s) |
| Terminator BamHI cutsite 6xHist tag EcnB TEV cutsite EcnA NdeI cutsite RBS T7 | 396,438 390,395 372,389 228,371 207,227 87,206 81,86 22,80 1,21 | feature/misc sequence_feature feature/misc sequence_feature tag feature/tag CDS feature/cds sequence_feature feature/misc CDS feature/cds sequence_feature feature/misc ribosome_entry_site feature/rbs feature/promoter promoter |