Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Veronica Pinamonti
Date created: 2015-08-19 11:00:00
Date modified: 2015-09-17 05:19:54
araC-pBAD + β
-carotene + J23100 + blh
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1604022_sequence (Version 1) |
Description
This biobrick is obtained by the assembly of two parts K1604020 and K1604021. The composite part contains the five genes necessary for the retinal synthesis. The first part contains ctrEIBY under araC-pBAD, these four gene are responsive of beta-carotene production starting from FPP, a colurless precurse presents in E.coli. The second part contains blh under costitutive promoter. It encodes for the beta-carotene15-15'dioxygenase that catalizes the cleavege of a single molecule of beta-carotene into two molecules of retinal.Notes
The two parts was joined together by strandard assembly, before it beta-carotene was amplified by Phusion PCR.Source
AraC-pBAD promoter comes from E.coli genome, while ctrEIBY are from Pantoea ananatis (formerly Erwinia uredovora) genome. Blh gene and its promoter are synthetized and codon optimezed by GeneScript.| Access | Instance | Definition |
| public public | BBa_K1604021 BBa_K1604020 | BBa_K1604021 BBa_K1604020 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1604021 BBa_K1604020 | 1,889 898,6663 | BBa_K1604021 BBa_K1604020 |