BBa_K1605009

BBa_K1605009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1605009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mike Toomey

Date created: 2015-09-13 11:00:00

Date modified: 2015-09-14 04:31:04

Ptet mRFP nifS RBS

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1605009_sequence (Version 1)

Description

Used BBa_I13531 as template and replaced part B0034 with artificial RBS for nifS

Notes

Had to take into consideration desired translation initiation rate

Source

Salis Lab RBS calculator

Components

Access	Instance	Definition
public	BBa_R0040	p(tetR)
public	BBa_K1605008	BBa_K1605008
public	BBa_E1010	mRFP1
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040	1,54	p(tetR)
BBa_K1605008	63,77	BBa_K1605008
BBa_E1010	84,789	mRFP1
BBa_B0010	798,877	BBa_B0010
BBa_B0012	886,926	BBa_B0012

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1605009/1

Member of these Collections

• iGEM Parts Registry