Types | DnaRegion
|
Roles | engineered_region
Composite
|
Sequences | BBa_K1606001_sequence (Version 1)
|
Description
Taking kumamolisin and putting it in a vector of yeast that has alpha secretion factor then putting the plasmid in yeast. The yeast will produce and secrete kumamolisin enzyme to its external environment and will destroys gluten while the bread continues to be fermented.
Notes
"Point mutations at residues 291, 319, 358, and 368 from Asparagine to Aspartate, Glycine to Serine, Aspartate to Glycine, and Aspartate to Histidine, respectively." and adding it into alpha secretion factor.
Source
Original protein comes form Alicyclobacillus sendaiensis. (Genetically engineered.)