Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lee, Chang-Lung
Date created: 2015-09-04 11:00:00
Date modified: 2015-09-05 08:08:17
K1613017 is a new part which can detect lead (Pb) ions in the liquid.
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1613017_sequence (Version 1) |
Description
This part is desinged to proceed lead detection in e.coli. When lead ions (Pb2+) enter the e.coli, they will combine with the protein PbrR and then form a dimer. The lead promoter can be controlled by the dimer to further trigger the RFP.The idea of this design came from Brown 2007 igem team, also working on lead detection. We combined I721001 and I721002 from their teams with a constitutive promoter J23102 and RFP to make them function.
Notes
At first, we used I721001 and I721002 from 2012 kit plate, but then we found out that they were inconsistent because of the failed transformation we did. So we got the sequences of these two parts from genebank and ordered these two parts.Source
The sequence was retrieved from genebank.| Access | Instance | Definition |
| public public public public public public public public | BBa_J23102 BBa_B0032 BBa_I721002 BBa_B0015 BBa_I721001 BBa_B0034 BBa_E1010 BBa_B0015 | BBa_J23102 BBa_B0032 BBa_I721002 BBa_B0015 BBa_I721001 BBa_B0034 mRFP1 BBa_B0015 |