H/E-rev

BBa_K1616005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1616005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Johanna Chesnel

Date created: 2015-09-16 11:00:00

Date modified: 2015-09-22 07:07:55

Holin/endolysin reversed - RBS and double T7 terminator

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1616005_sequence (Version 1)

Description

Holin and endolysin are toxins; after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bl??si, 1995)

Notes

Check of illegal sites

Source

BBa_K1616005 is a composite part with BBa_K1616006 (endolysin reversed), BBa_K1616007 (reversed), BBa_K1616011 (RBS reversed) and BBa_B0025 (Double terminator T7 reversed).

Components

Access	Instance	Definition
public	BBa_B0025	BBa_B0025
public	BBa_K1616006	End-rev
public	BBa_K1616011	RBS-rev
public	BBa_K1616007	Holin-rev
public	BBa_K1616011	RBS-rev

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0025	1,129	BBa_B0025
BBa_K1616006	138,651	End-rev
BBa_K1616011	660,673	RBS-rev
BBa_K1616007	682,1357	Holin-rev
BBa_K1616011	1366,1379	RBS-rev

Other Properties

• igem#experience: Issues
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1616005/1

Member of these Collections

• iGEM Parts Registry