Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nurgeldi Bazarov
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-15 12:46:07
mammalian LacI with miR-223 and miR-21 binding sites
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1639011_sequence (Version 1) |
Description
In our project second step of cancer module is based on production of LacI so that it will suppress expression of DsRed. Binding site on 3' end of LacI makes it regulatable and limits it's expression only in cells with low miR-223 and miR-21 levels. miR-21 has high rate of production in most cancer types. In gastric epithelial cells level of this miRNAs are low but in gastric cancer cells their level is very high.Notes
We suggest also to view similar part we submitted-BBa_K1639010. It contains mLacI with miR binding sites but have restriction enzyme cut sites to remove miR binding site as needed.Source
This protein is mammalian codon optimized version of LacI repressor originally derived from E.coli. It also possess nuclear localization signal (NLS) so that it can act on lac operators in nucleus.| Sequence Annotation | Location | Component / Role(s) |
| Kozak ATG mLacI SV40 NLS Stop Codon miR-223 binding site miR-21 binding site | 1,6 7,9 7,1125 1102,1122 1123,1128 1129,1150 1180,1200 | feature/rbs ribosome_entry_site start_codon feature/start feature/protein CDS feature/tag tag stop_codon feature/stop non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding |