Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Leung King Pong
Date created: 2015-07-15 11:00:00
Date modified: 2015-09-16 08:39:46
Recombination Template for mamAB Operon with Promotor and Terminator
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1648002_sequence (Version 1) |
Description
Consist of a template for recombination of mamAB operon(K1648000), a template for recombination of mamXY, mamGC and mms operon(K1648001), a promotor & RBS(J13002) in front of each template and a double terminator(B0015) at the back of each template.This part is designed for homologous recombination to introduce the large magnetosome forming operon into Azotobactor vinelandii.
This part is introduced into A. vinelandii genome via random integration before full operon sequences to be transformed into A. vinelandii.
Notes
We add a promotor and RBS before each template to initial the coding and a double terminator(B0015) behind each template to terminate the coding and ligate these parts together.Source
Magnetospirillum gryphiswaldense MSR-1 v2, complete genome| Access | Instance | Definition |
| public public public | BBa_J13002 BBa_K1648000 BBa_B0015 | BBa_J13002 BBa_K1648000 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J13002 BBa_K1648000 BBa_B0015 | 1,74 81,1100 1109,1237 | BBa_J13002 BBa_K1648000 BBa_B0015 |