Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Annakarin Korppoo
Date created: 2015-09-13 11:00:00
Date modified: 2015-09-14 12:59:13
Propane 1 codes three of the ten enzymes necessary to produce propane in Escherichia coli.
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1655000_sequence (Version 1) |
Description
Propane 1 includes 1)acyl-CoA thioester hydrolase (YciA) from Haemophilus influenzae, which catalyzes the reaction: acyl-CoA + H2O <-> CoA + a carboxylate, 2) Carboxylic acid reductase (CAR) from Mycobacterium marinum (strain ATCC BAA-535 / M), which catalyzes the reaction: a carboxylate + reduced acceptor <-> an aldehyde + acceptor + H2O, and 3) maturation factor phosphopantetheinyl transferase (sfp) from Bacillus subtilis, which activates CAR by catalyzing the transfer of a prosthetic phosphopanteine group to CAR.All coding regions are assembled as an operon after a T7 promoter and and RBS from common cloning vectors (such as pCDF-Duet1 by Novagen). Terminator sequence after the last coding sequence, CAR is also derived from common cloning vectors (e.g. pCDF-Duet1 by Novagen uses this terminator).
All coding sequences have been codon optimized twice for E. coli. First with Thermo Fisher's codon optimizer and then with IDT's codon optimization software.
Notes
Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.Source
The part sources are Haemophilus influenzae, Mycobacterium marinum (strain ATCC BAA-535 / M), and Bacillus subtilis for YciA, CAR and sfp, respectively. The promoter, RBS, and terminator sequences are derived from the commercial vector pCDF-Duet1 by Novagen.| Sequence Annotation | Location | Component / Role(s) |
| T7 Lac RBS YciA RBS Sfp RBS CAR T7 double terminator | 1,20 22,43 76,82 90,555 579,585 593,1268 1283,1289 1296,4821 4856,4903 | promoter feature/promoter feature/operator operator feature/rbs ribosome_entry_site feature/cds CDS feature/rbs ribosome_entry_site feature/cds CDS ribosome_entry_site feature/rbs CDS feature/cds feature/misc sequence_feature |