Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Janet Standeven Jack Kwon
Date created: 2015-08-25 11:00:00
Date modified: 2015-09-13 05:21:21
Construct 1 Bicistronic:PelB-CDA under tetR and GFP under P(Lac)IQ promoter
| Types | DnaRegion |
| Roles | engineered_region Project |
| Sequences | BBa_K1662000_sequence (Version 1) |
Description
pelB tag for localization to the periplasm with Chitin deacetylaseNotes
We had to eliminate an illegal restriction site within the mature CDA protein. pel B was also problematic due to hairpin structures that were likely to form. We engineered these by base pair substitutionsSource
saccharomyces cerevisiae- CDA2 mature protein, with a pelB sequence| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 TetR Promoter scar BBa_B0030 RBS Start codon BBa_J32015 PelB Mature CDA2 Double stop codons BBa_I14032 P(Lac) IQ Promoter scar BBa_B0030 RBS Start codon eGFP Double stop codons BBa_B0015 Terminator | 1,54 55,60 88,102 110,112 113,175 176,979 980,985 986,1022 1023,1028 1056,1070 1078,1080 1081,1794 1795,1800 1801,1929 | promoter feature/promoter feature/scar feature/rbs ribosome_entry_site start_codon feature/start sequence_feature feature/misc sequence_feature feature/misc feature/stop stop_codon feature/promoter promoter feature/scar ribosome_entry_site feature/rbs feature/start start_codon feature/misc sequence_feature stop_codon feature/stop sequence_feature feature/misc |