Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jack Kwon
Date created: 2015-09-03 11:00:00
Date modified: 2015-09-14 11:15:25
Construct 3 Fusion: TetR Promoter:PelB-CDA-eGFP
| Types | DnaRegion |
| Roles | engineered_region Project |
| Sequences | BBa_K1662002_sequence (Version 1) |
Description
The purpose of this construct is to produce a single mRNA transcript that it can be translated into one fusion protein. This protein will be led into the periplasm of E. Coli because the PelB leader sequence leads the protein into the periplasm. Th PelB leader sequence leads the protein into the perpiplasm to keep the expression of the CDA gene from killing the E. Coli. The eGFP gene is there to allow the lab workers to see physically (green fluorescent light) that the mRNA transcript has been successfully translated and that the genes are being expressed correctly.Notes
Wobble introduced to eliminate illegal restriction sites.Source
Mature CDA2 comes from saccharomyces cerevisiae. PelB sequence was pulled from the iGEM registry.| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 TetR Promoter scar BBa_B0030 RBS Start codon BBa_J32015 PelB Mature CDA2 eGFP Double stop codon BBa_B0015 terminator | 1,54 55,60 88,102 110,112 113,175 176,979 980,1693 1694,1699 1700,1828 | feature/promoter promoter feature/scar ribosome_entry_site feature/rbs start_codon feature/start feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc stop_codon feature/stop feature/misc sequence_feature |