BBa_K1675005

BBa_K1675005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1675005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: JIAQI XU
Date created: 2015-09-09 11:00:00
Date modified: 2015-09-12 12:25:21

J23119+B0034+NhaB(BBa_K1675001)



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1675005_sequence (Version 1)

Description

The constitutive promoter J23119, the ribosome binding site B0034 and the functional gene nhaB were used in this new standard part. This part is available for the bacteria to survive in alkaline environment.

Notes

mutation of CTG to CTA Leu.(site:383bp); mutation of GCT to GCG Ala.(site:461bp); mutation of ACC to ACT Thr.(site:566bp);
mutation of GAC to GAT Asp.(site:572bp); mutation of ACC to ACT Thr.(site:677bp); mutation of GTA to GTT Val.(site:692bp); mutation of GCA to GCG Ala.(site:1040bp); mutation of GAG to GAA Glu.(site:1067bp); mutation of GTC to GTA Val.(site:1499bp)

Source

The sequence of promoter J23119 is found through the standard part BBa_J23119 http://parts.igem.org/Part:BBa_J23119
The sequence of ribosome binding site B0034 is found through the standard part BBa_B0034 http://parts.igem.org/Part:BBa_B0034
The sequence of gene NhaB is found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=1233176&to=1234717&strand=true

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1675005/1