Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: JIAQI XU
Date created: 2015-09-11 11:00:00
Date modified: 2015-09-12 02:10:42
J23119+B0034+NhaA+J23119+B0034+NhaB
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1675015_sequence (Version 1) |
Description
Construct the JBA (J23119+B0034), B0034 and nhaB with the vector pSB1C3. Two functional genes nhaA and nhaB are used to pump H+ in and lower the pH of bacteria???s microenvironment. JBA and JBB are linked with pSB1C3 through 3A Assemble successfully. Thus, it can be an alkaline-resisting part.Notes
mutation of CTG to CTA Leu.(site:1552bp); mutation of GCT to GCG Ala.(site:1630bp); mutation of ACC to ACT Thr.(site:1780bp); mutation of GAC to GAT Asp.(site:1786bp); mutation of ACC to ACT Thr.(site:1891bp); mutation of GTA to GTG Val.(site:1906bp); mutation of GCA to GCG Ala.(site:2254bp); mutation of GAG to GAA Glu.(site:2281bp); mutation of GTC to GTA Val.(site:2713bp)Source
The gene NhaA and NhaB come from Escherichia coli str. K-12. The J23119 promoter and the B0034 RBS come from the standard parts.| Access | Instance | Definition |
| public public public public public public | BBa_J23119 BBa_B0034 BBa_K1675000 BBa_J23119 BBa_B0034 BBa_K1675001 | BBa_J23119 BBa_B0034 BBa_K1675000 BBa_J23119 BBa_B0034 BBa_K1675001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23119 BBa_B0034 BBa_K1675000 BBa_J23119 BBa_B0034 BBa_K1675001 | 1,35 44,55 64,1230 1239,1273 1282,1293 1300,2841 | BBa_J23119 BBa_B0034 BBa_K1675000 BBa_J23119 BBa_B0034 BBa_K1675001 |