BBa_K1676049

BBa_K1676049 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1676049
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ooi Kean Hean
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-16 05:23:40

GFP Reporter with Mutant RBS 5AC



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_K1676049_sequence (Version 1)

Description

This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the second base pair. The FIFTH base pair A is substituted to G. The construct consist of the pLac promoter BBa_R0011, mutant RBS, eGFP BBa_E0040 and double terminator BBa_B0015.
The purpose of this construct is to characterise the mutant RBS in Shewanella Oneidensis MR1 strain.

Notes

pLac was used as it is a constitutive promoter in S. Oneidensis MR1.

Source

The basic parts are from the iGEM registry. Consisting of the pLac promoter BBa_R0011, eGFP BBa_E0040, double terminator BBa_B0015, we ligated the parts( including original wild type RBS) together and perform site targeted mutagenesis on the construct to mutate the WT RBS.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1676049/1