Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ooi Kean Hean
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-16 05:23:40
GFP Reporter with Mutant RBS 5AC
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K1676049_sequence (Version 1) |
Description
This reporter carries a mutant of RBS BBa_B0034. This mutant carries a single base pair substitution on the second base pair. The FIFTH base pair A is substituted to G. The construct consist of the pLac promoter BBa_R0011, mutant RBS, eGFP BBa_E0040 and double terminator BBa_B0015.The purpose of this construct is to characterise the mutant RBS in Shewanella Oneidensis MR1 strain.
Notes
pLac was used as it is a constitutive promoter in S. Oneidensis MR1.Source
The basic parts are from the iGEM registry. Consisting of the pLac promoter BBa_R0011, eGFP BBa_E0040, double terminator BBa_B0015, we ligated the parts( including original wild type RBS) together and perform site targeted mutagenesis on the construct to mutate the WT RBS.| Access | Instance | Definition |
| public public public public | BBa_R0011 BBa_K1676013 BBa_E0040 BBa_B0015 | lacI+pL BBa_K1676013 GFP BBa_B0015 |