BBa_K1676096

BBa_K1676096 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1676096
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ooi Kean Hean
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 10:04:39

Mutant 20 of LDH and wildtype LDP



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Composite

    Sequences BBa_K1676096_sequence (Version 1)

    Description

    This construct is an operon for expression of our mutant LDH and wildtype LDP.

    Notes

    Primers were designed to have the start and stop codon so that they will not be mutated during PCR

    Source

    Wild type LDH and LDP was obtained from a Pseudomonas Strain and random mutagenesis was carried out by PCR to make lots of Mutant LDH. PCR fragments carrying the mutants were then ligated to the wild type LDP to make a complete operon.

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1676096/1
     

    Attachments

    © 2018 Newcastle University, University of Utah, and collaborators
    About SynBioHub | View Source on Github | Report an Issue
    | v1.6.1 (86615526)