Types | DnaRegion
|
Roles | Regulatory
promoter
|
Sequences | BBa_K1679004_sequence (Version 1)
|
Description
BBa_J23101 is a strong promoter which is well characterized by many teams who take part in 2015 InterLab Study. RiboJ is a Ribozyme-based insulator part that can buffer synthetic circuits from genetic context. This part can be used as a promoter-like parts.
Notes
We want to make a promoter part containing RiboJ to buffer synthetic circuits from genetic context. We synthesized RiboJ with EXSP site and use 3A Assembly to construct this part.
Source
BBa_J23101
We synthesized RiboJ. Our reference is Lou C, Stanton B, Chen Y J, et al. Ribozyme-based insulator parts buffer synthetic circuits from genetic context[J]. Nature biotechnology, 2012, 30(11): 1137-1142.