BBa_K1679004

BBa_K1679004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1679004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Qikai Qin
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-18 06:26:36

J23101 and RiboJ



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1679004_sequence (Version 1)

Description

BBa_J23101 is a strong promoter which is well characterized by many teams who take part in 2015 InterLab Study. RiboJ is a Ribozyme-based insulator part that can buffer synthetic circuits from genetic context. This part can be used as a promoter-like parts.

Notes

We want to make a promoter part containing RiboJ to buffer synthetic circuits from genetic context. We synthesized RiboJ with EXSP site and use 3A Assembly to construct this part.

Source

BBa_J23101
We synthesized RiboJ. Our reference is Lou C, Stanton B, Chen Y J, et al. Ribozyme-based insulator parts buffer synthetic circuits from genetic context[J]. Nature biotechnology, 2012, 30(11): 1137-1142.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1679004/1