BBa_K1680026

BBa_K1680026 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1680026

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Katharina Sporbeck

Date created: 2015-09-17 11:00:00

Date modified: 2015-10-28 03:58:14

pETUE

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_K1680026_sequence (Version 1)

Description

Expression Plasmid coding for His-tag, also containing a thrombine cleavage site between coding region and Histag, so that His-tag can be cleaved off. Vecotr opening with PstI/XmaI, clone part into it with PstI/NgoMIV.
Ampicillin resistance.

Notes

very strong RBS
6xHis-Tag
Thromin-cleavage-site
NgoMIV-compatible restriction site for in-frame insertion

Source

pET-Vector

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
T7 promoter	1,19	feature/promoter, promoter
Lac operator	20,44	operator, feature/operator
RBS.1 strong	53,67	feature/rbs, ribosome_entry_site
6x His-tag	77,94	feature/tag, tag
thrombin cleavage site	104,121	feature/misc, sequence_feature
tet promoter	212,240	feature/promoter, promoter
rrnB T1 terminator	410,496	stop_codon, feature/stop
T7 terminator	683,730	feature/stop, stop_codon
f1 ori	767,1222	feature/misc, sequence_feature
AmpR	1337,2197	feature/misc, sequence_feature
ori	2290,2878	feature/misc, sequence_feature
lac operator	3277,3296	feature/operator, operator

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1680026/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //plasmid
• //plasmid/expression
• //plasmid/expression/t7
• //plasmidbackbone/copynumber/high
• //plasmidbackbone/expression/inducible
• iGEM Parts Registry
• igem_plasmid_vectors