Dioxy

BBa_K1688022 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1688022
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Delyan Georgiev
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-18 11:10:54

Catechol 1,2 Dioxygenase + HlyA export tag



Types
DnaRegion

Roles
engineered_region

Intermediate

Sequences BBa_K1688022_sequence (Version 1)

Description

Catechol 1,2 dioxygenase (BBa_K1092003) fused to HlyA export tag (BBa_K554002), but with an introduced frameshift due to improper assembly.

Notes

Primers for restriction-free cloning were designed in such a way as to remove the STOP codon of the catechol 1,2 dioxygenase and the scar left from 3A assembly. However, due to a design error, an AG insert between the two parts was introduced, resulting in a stop codon at the beginning of the HlyA sequence.

Source

Restriction free cloning.

Sequence Annotation Location Component / Role(s)
T7 Promoter
Dioxygenase
START
HlyA C-tag
STOP
STOP
Frameshift insertion
RBS
3A Scar
1,20
39,971
39,41
974,1153
1154,1156
1157,1159
972,973
21,32
33,38
feature/promoter promoter
CDS feature/protein
feature/start start_codon
tag feature/tag
stop_codon feature/stop
feature/stop stop_codon
feature/mutation sequence_alteration
ribosome_entry_site feature/rbs
feature/scar
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1688022/1