Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Meixi Liu
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 09:57:13
Use toehold to regulate GFP
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K1700000_sequence (Version 1) |
Description
The structure of toehold switch is similar to hairpin, except it has a loop at the top as ???toehold???. Toehold switch functions as riboregulator through linear-linear interaction between RNAs. When target RNA appears, it will bind one of the toehold switch stems and open the loop, exposing the RBS.Toehold switch systems are composed of two RNA strands referred to as the switch and trigger. The switch RNA contains the coding sequence of the gene being regulated. Upstream of this coding sequence is a hairpin-based processing module containing both a strong RBS and a start codon that is followed by a common 21 nt linker sequence coding for low-molecular-weight amino acids added to the N terminus of the gene of interest. A single-stranded toehold sequence at the 50 end of the hairpin module provides the initial binding site for the trigger RNA strand. This trigger molecule contains an extended single-stranded region that completes a branch migration process with the hairpin to expose the RBS and start codon, thereby initiating translation of the gene of interest.
The trigger RNA of our toehold is miRNA-144.When adding it to the system, GFP can be expressed.
Notes
Toehold can be open by trigger RNA, thus letting GFP express.Source
We synthesis the part.| Sequence Annotation | Location | Component / Role(s) |
| toehold ATG start codon GFP coding sequence TAA stop codon | 1,100 156,158 101,820 818,820 | sequence_feature feature/misc feature/start start_codon CDS feature/cds stop_codon feature/stop |