Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Emilie Cuillery
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-15 08:53:46
sgRNA Z4 expressing cassette
| Types | DnaRegion |
| Roles | mature_transcript_region RNA |
| Sequences | BBa_K1723003_sequence (Version 1) |
Description
This part is expressing the sgRNA (single guide RNA) Z4 that can bind to dCas9-ω (BBa_K1723000). The complex was designed to bind the PAM rich URS J23117 promoter (BBa_K1723001) to activate the production of the target gene linked to the promoter. This part is a part of a gene regulation system built with dCas9-ω, with the parts sgRNA Z0 expressing cassette (BBa_K1723002) and gRNA Z35 expressing cassette (BBa_K1723004). This sgRNA coding sequence was biobricked with the promoter pBAD and its terminator, both used for our experiments. As the gRNA sequence has to start the first base after the promoter it couldn't have been used with the standard BioBrick system so the promoter is provided already. It is possible to PCR out the part to put it under another promoter, but he terminator is specific and it is recommended to keep it.Notes
This part was design on the model for sgRNAs on the paper from Alec AK Nielsen & Christopher A Voigt [3] and the specific sequence for the gRNA Z4 was designed from the sequence of the plasmid pWJ89 D.Bikard [1] sent us.Source
This sequence was fully synthesized.| Sequence Annotation | Location | Component / Role(s) |
| pBAD promoter Z4 specific sequence sgRNA scaffold S. Pyogenes terminator | 1,173 174,193 194,235 236,275 | feature/promoter promoter sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature |