Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Emilie Cuillery
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-15 10:48:08
PAM rich URS J23117Alt promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1723005_sequence (Version 1) |
Description
PAM rich URS J23117 is a promoter made from the existing PAM rich URS J23117 promoter (BBa_K1723001) and mutated. This promoter has PAM (PAM = NGG sequence) rich Upstream Regulatory Sequence to enable the use of protein dCas9-ω (BBa_K1723000) as a gene transcription regulator when in complex with one sgRNA targeting the promoter such as: X0 (BBa_K1723006), X4 (BBa_K1723007), or X35 (BBa_K1723008).Discover all the parts that can work with this one:
http://2015.igem.org/Team:EPF_Lausanne/Part_Collection
Notes
This part was synthesized on the model of the PAM rich URS J23117 promoter. It was obtained by mutating bases on the promoter to obtain different target sites for sgRNAs for promoter regulation using dCas9-ω system.Source
this part was fully synthesized.| Sequence Annotation | Location | Component / Role(s) |
| PAM rich URS J23117Alt PAM sgRNA X4 recognition site PAM sgRNA X35 recognition site PAM sgRNA X0 recognition site -10 sequence -35 sequence | 1,247 248,282 189,191 192,211 242,244 245,264 269,271 249,268 271,276 248,253 | feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature |