BBa_K1725002

BBa_K1725002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1725002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mhairi Davidson

Date created: 2015-08-10 11:00:00

Date modified: 2016-07-23 11:17:58

PhlF repressible promoter + weak RBS + GFP

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K1725002_sequence (Version 1)

Description

The promoter is on normally, and off when the PhlF repressor (K1725041) is bound to it. We measured the strength of the promoter with GFP and two different ribosome binding sites (B0032 and B0034), and compared to R0040 and K1725020.

Notes

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Source

PhlF promoter from "Genomic Mining of Prokaryotic Repressors for Orthogonal Logic Gates" Stanton et al http://www.nature.com/nchembio/journal/v10/n2/full/nchembio.1411.html

Components

Access	Instance	Definition
public	BBa_K1725000	P-phlF
public	BBa_E5501	BBa_E5501

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1725000	1,48	P-phlF
BBa_E5501	57,795	BBa_E5501

Other Properties

• igem#experience: Works
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1725002/1

Member of these Collections

• iGEM Parts Registry