BBa_K1725421

BBa_K1725421 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1725421

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andrey Filipov

Date created: 2015-09-07 11:00:00

Date modified: 2016-01-25 11:31:22

RBS + UirR

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K1725421_sequence (Version 1)

Description

The regulator (K1725420) that participates together with PlsiR (K1725400) and UirS (K1725410) to activate a response to UV-A light has been put downstream of the RBS B0032.

Notes

Due to the length of the DNA sequence, the gene was amplified via PCR and the BioBrick suffix and prefix, alongside with the RBS, were added as part of the primers. The last base of the gene was changed from G to A because different stop codons can be more efficient in different species and TAA is especially efficient in E. coli.

Source

UirR comes from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803.

Components

Access	Instance	Definition
public	BBa_B0032	BBa_B0032
public	BBa_K1725420	UirR

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0032	1,13	BBa_B0032
BBa_K1725420	20,745	UirR

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1725421/1

Member of these Collections

• iGEM Parts Registry