Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrey Filipov
Date created: 2015-09-07 11:00:00
Date modified: 2015-09-08 01:22:59
J23110 + RBS + UirR + B0015 + PlsiR + GFP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1725431_sequence (Version 1) |
Description
The UV intensity response regulator coding sequence was positioned downstream of J23110, a constitutive promoter family member, and upstream of the double terminator B0015. In this part the promoter associated with UirR was also added, alongside with GFP, so that activity may be reported.Notes
J23110, UirR, and B0015 were ligated to Plsir and GFP after restriction that was followed by an Azure-A-based gel extraction.Source
UirR and Plsir come from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803.| Access | Instance | Definition |
| public public | BBa_K1725427 BBa_K1725401 | BBa_K1725427 PlsiR.GFP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1725427 BBa_K1725401 | 1,925 934,2213 | BBa_K1725427 PlsiR.GFP |