BBa_K1725432

BBa_K1725432 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1725432

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andrey Filipov

Date created: 2015-09-07 11:00:00

Date modified: 2015-09-08 01:22:54

J23114 + RBS + UirR + B0015 + PlsiR + GFP

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1725432_sequence (Version 1)

Description

The UV intensity response regulator coding sequence was positioned downstream of J23114, a constitutive promoter family member, and upstream of the double terminator B0015. In this part the promoter associated with UirR was also added, alongside with GFP, so that activity may be reported.

Notes

J23114, UirR, and B0015 were ligated to Plsir and GFP after restriction that was followed by an Azure-A-based gel extraction.

Source

UirR and Plsir come from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803.

Components

Access	Instance	Definition
public	BBa_K1725428	BBa_K1725428
public	BBa_K1725401	PlsiR.GFP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1725428	1,925	BBa_K1725428
BBa_K1725401	934,2213	PlsiR.GFP

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1725432/1

Member of these Collections

• iGEM Parts Registry