Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ying Kuan
Date created: 2015-09-04 11:00:00
Date modified: 2016-02-03 10:05:22
Lac operator+RBS+Signal peptide+Signiferin+Terminator
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1727006_sequence (Version 1) |
Description
Antimicrobial peptides (AMPs) are stable peptides that have extensive ability to kill or inhibit the growth of bacteria. They play a role in defense mechanism for all organisms, ranging from prokaryotes to humans, to against predators. Some of them act against specific strain of microbes.Signiferin is a kinds of antimicrobial peptide extracted from the skin mucus of Crinia signifera, respectively. It demonstrated effectiveness in killing MRSA. And we had change it's sequence into E.coli codon usage. We treat a signal peptide in front of signiferin. This signal peptide comes from S.lividas and has ability to let E.coli secrect signiferin to LB culture medium.
We used Lac operator,RBS,terminator on pQE60 to made our expression machine worked.
Notes
PQESource
Signiferin with signal peptide is artificial synthesis.| Access | Instance | Definition |
| public | BBa_K1727000 | BBa_K1727000 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1727000 | 1,179 | BBa_K1727000 |