Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ying Kuan
Date created: 2015-09-04 11:00:00
Date modified: 2016-02-01 01:24:01
T5+Lac Operator+RBS+signal peptide+Epinecidin-1+Terminater
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1727007_sequence (Version 1) |
Description
Antimicrobial peptides (AMPs) are stable peptides that have extensive ability to kill or inhibit the growth of bacteria. They play a role in defense mechanism for all organisms, ranging from prokaryotes to humans, to against predators. Some of them act against specific strain of microbes.Epinecidin-1 is a kind of antimicrobial peptide. This AMP is extracted from the skin mucus of Epinephelus coioides, respectively. Epinecidin-1 has been shown to help wound healing.We treat a signal peptide in front of Epinecidin-1. This signal peptide comes from S.lividas and has ability to let E.coli secrect signiferin to LB culture medium.
We use promoter,Lac operator,RBS,terminator on pQE 60 vector.
Notes
NoSource
Epicidni| Sequence Annotation | Location | Component / Role(s) |
| T5 promoter -35 Lac operator -10 Lac operator RBS Restriction enzyme BamH I site Signal peptide Restriction enzyme Bgl II site Epinecidin-1 Alanine cutting site lambda t0 terminator | 1,45 16,21 21,37 39,45 53,69 92,97 112,117 118,207 415,420 211,414 208,210 461,555 | promoter feature/promoter feature/dna sequence_feature feature/operator operator sequence_feature feature/dna feature/operator operator ribosome_entry_site feature/rbs feature/misc sequence_feature feature/cds CDS feature/misc sequence_feature CDS feature/protein feature/cds CDS stop_codon feature/stop |