Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ying Kuan
Date created: 2015-09-04 11:00:00
Date modified: 2016-02-03 10:43:43
T5+Lac Operator+RBS+Signal peptide+Signiferin+Terminater
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1727008_sequence (Version 1) |
Description
Antimicrobial peptides (AMPs) are stable peptides that have extensive ability to kill or inhibit the growth of bacteria. They play a role in defense mechanism for all organisms, ranging from prokaryotes to humans, to against predators. Some of them act against specific strain of microbes.Signiferin is a kinds of antimicrobial peptide extracted from the skin mucus of Crinia signifera, respectively. It demonstrated effectiveness in killing MRSA. And we had change it's sequence into E.coli codon usage. We treat a signal peptide in front of signiferin. This signal peptide comes from S.lividas and has ability to let E.coli secrect signiferin to LB culture medium.
We used promoter,Lac operator,RBS,terminator on pQE60 to made our expression machine worked.
Notes
NoSource
EPi| Sequence Annotation | Location | Component / Role(s) |
| T5 promoter -35 -10 Lac operator RBS Lac operator Restriction enzyme Nco I site Signal peptide Alanine cutting site Signiferin 3X stop codon Restriction enzyme BamH I site lambda t0 terminator | 1,45 16,21 39,45 53,69 92,97 21,37 104,109 106,195 196,198 199,243 244,252 253,258 305,399 | feature/promoter promoter sequence_feature feature/dna sequence_feature feature/dna operator feature/operator feature/rbs ribosome_entry_site feature/operator operator sequence_feature feature/misc feature/cds CDS CDS feature/cds CDS feature/protein stop_codon feature/stop feature/misc sequence_feature feature/stop stop_codon |