Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Donna Lee
Date created: 2015-09-06 11:00:00
Date modified: 2015-09-18 12:54:16
pSB1C3-J23100-B0034-CDcel-Gaussia-His-B0015
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_K1732004_sequence (Version 1) |
Description
J23100 constitutive promoter (BBa_J23100), a RBS (BBa_B0034), CDcel domain (BBa_K1732002), Gaussia luciferase (BBa_K1732003), and B0015 terminator (BBa_B0015).This DNA sequence, expressed from a constitutive, strong promoter allows for the Gaussia luciferase protein to be synthesized and was targeted extrecellularly into the media. The Gaussia luciferase proteins were reacted with the optimal concentration of coelenterazine to express luminescence, which was used to test the Carnegie Mellon DIY luminometer.
Notes
E.coli was codon optimized for E.coli.Source
Sequence was obtained from Cheryl Telmer from the Bruchez lab in Carnegie Mellon University.| Access | Instance | Definition |
| public public public public public | BBa_J23100 BBa_B0034 BBa_K1732002 BBa_K1732027 BBa_B0015 | BBa_J23100 BBa_B0034 BBa_K1732002 BBa_K1732027 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_K1732002 BBa_K1732027 BBa_B0015 | 1,35 44,55 64,1098 1107,1628 1637,1765 | BBa_J23100 BBa_B0034 BBa_K1732002 BBa_K1732027 BBa_B0015 |