Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: The Newcastle 2009 iGEM team
Date created: 2009-10-03 11:00:00
Date modified: 2015-05-08 01:10:58
Heritable, Tunable, Stochastic Switch
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K174003_sequence (Version 1) |
Description
This tunable switch is biased heads or tails. It can be tuned to control cell differention and fate stochastically.Notes
It can be tuned by IPTG and Xylose however if one wishes to control the switch with something else, the control regions can easily be replaced since they are surrounded with restriction sites.Source
Synthesised| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0014 BBa_E1010 (reverse) RBS from pmutin4_lacZ (reverse) AccIII Cut Site LacI binding site (oid) promoter from pmutin4_lacZ BalI Cut Site BBa_J44000 BBa_K143012 sigA promoter (pveg-reverse) RBS from pmutin4_lacZ BBa_J31001 Hin CDS ssrA ALGG degradation tag Hin stop codon BBa_B0014 Double Terminator BBa_J44000 HixC NaeI Cut Site xylA promoter (reverse) NruI Cut Site | 1,95 96,776 777,793 794,799 883,896 800,905 906,911 912,937 938,1034 1035,1051 1052,1621 1622,1666 1667,1669 1670,1764 1765,1790 1791,1796 1797,1922 1923,1928 | feature/BioBrick engineered_region feature/BioBrick engineered_region ribosome_entry_site feature/rbs feature/misc sequence_feature non_covalent_binding_site feature/binding promoter feature/promoter sequence_feature feature/misc engineered_region feature/BioBrick feature/BioBrick engineered_region feature/rbs ribosome_entry_site feature/BioBrick engineered_region non_covalent_binding_site feature/binding feature/stop stop_codon feature/BioBrick engineered_region engineered_region feature/BioBrick sequence_feature feature/misc feature/promoter promoter sequence_feature feature/misc |