Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: The Newcastle 2009 iGEM team
Date created: 2009-10-13 11:00:00
Date modified: 2015-05-08 01:10:58
cwlJ + sleB, B. subtilis germination genes
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K174014_sequence (Version 1) |
Description
Both CwlJ and SleB are germination specific cortex hydrolases. Although SleB has a major effect for the germination, cwlJ's effect is minor. However bacillus subtilis with double mutation without cwlJ and sleB were not able germinate again (2).To control the germination rate tightly, cwlJ and sleB genes are combined to make this device.
Notes
For tight regulation of germination, we decided to combine cwlJ and sleB.Source
B. subtilis| Access | Instance | Definition |
| public public | BBa_K174013 BBa_K174012 | BBa_K174013 BBa_K174012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K174013 BBa_K174012 | 1,441 450,1381 | BBa_K174013 BBa_K174012 |