Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marta Żardecka
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 04:48:49
sfGFP under a short arabinose promoter s2 without O1 and O2
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1741002_sequence (Version 1) |
Description
This, shortened version of araBAD promoter does not contain O1 and O2 regions, but still contains the CRP binding site.Notes
We removed O1 and O2 regions from the promoter.Source
All promoter fragments derive from Escherichia coli K12, strain DH5 alpha, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.| Sequence Annotation | Location | Component / Role(s) |
| CRP binding site I1, I2 binding site -35 signal -10 signal 5'UTR His-tag two consecutive STOP codons sfGFP | 21,31 23,65 63,67 87,91 101,127 128,157 872,877 158,871 | feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/tag tag stop_codon feature/stop CDS feature/protein |