Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marta Żardecka
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 05:30:32
sfGFP under melibiose promoter
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1741003_sequence (Version 1) |
Description
MelR chromosomal copy of MelR is sufficient to activate the melibiose melAB promoter copied from E. coli K12 chromosome when melibiose is added to M9 minimal medium or to a rich medium 2xLB (0,4%). In both media induction is much weaker than of arabinose or rhamnose induced promoters.Notes
None.Source
All promoter fragments derive from Escherichia coli K12, strain DH5 alpha, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.| Sequence Annotation | Location | Component / Role(s) |
| MelR binding site 1 CRP binding site MelR binding site 1 -35 signal -10 signal 5'UTR RBS His-tag sfGFP two consecutive STOP codons | 28,45 48,63 66,84 102,107 125,129 138,163 151,156 164,193 194,907 908,913 | non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/rbs ribosome_entry_site feature/tag tag feature/protein CDS stop_codon feature/stop |