Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marta Żardecka
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 07:24:06
sfGFP under shortened promoter xylA1 with improved 5'UTR
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1741009_sequence (Version 1) |
Description
Short version of xylA promoter with known functional elements is stronger than two other versions (BBa_K1741007, BBa_K1741008).Notes
None.Source
All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.| Sequence Annotation | Location | Component / Role(s) |
| CRP binding site XylR binding site XylR binding site -35 signal -10 signal modified 5'UTR part of 5'UTR taken from proD promoter His-tag sfGFP two consecutive STOP codons | 1,19 20,34 40,55 56,60 79,84 90,142 104,142 143,172 173,886 887,892 | feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/tag tag CDS feature/protein feature/stop stop_codon |