Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marta Żardecka
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-17 07:03:26
sfGFP under proC promoter with a hairpin in 5'UTR
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1741013_sequence (Version 1) |
Description
A weak hairpin introduced to 5???UTR slightly lowers transcription/translation. Will be extended to a thermometer, actually we see no temp. dependence.Notes
A weak hairpin introduced to 5'UTR.Source
All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.| Sequence Annotation | Location | Component / Role(s) |
| proC promoter -35 signal -10 signal 5'UTR with hairpin hairpin His-tag sfGFP two consectuive STOP codons | 1,101 63,68 86,91 102,189 145,175 190,219 220,933 934,939 | feature/promoter promoter sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/tag tag feature/protein CDS feature/stop stop_codon |