Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team Bielefeld-CeBiTec 2015
Date created: 2015-08-26 11:00:00
Date modified: 2015-09-18 06:41:46
RraA with N-terminal His-TEV-tag under T7 promoter
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1758122_sequence (Version 1) |
Description
With this part you can overproduce purify E. coli protein RraA. The part is made up of T7-promoter (BBa_I719005) and RraA fused to an N-terminal His-tag and TEV cleavage site.Source
This part was obtained from E. coli K12 genomic DNA and cloned to pSB1C3 with Gibson assembly.| Sequence Annotation | Location | Component / Role(s) |
| I719005 T7 RBS B0034 6xHis TEV cleavage site RraA (menG) B0010 B0012 | 1,23 1,23 32,43 32,43 62,79 101,118 125,619 628,707 716,756 | engineered_region feature/BioBrick feature/promoter promoter feature/rbs ribosome_entry_site engineered_region feature/BioBrick tag feature/tag feature/misc sequence_feature CDS feature/cds engineered_region feature/BioBrick engineered_region feature/BioBrick |