Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team Bielefeld-CeBiTec 2015
Date created: 2015-08-28 11:00:00
Date modified: 2015-09-18 05:53:38
His-tagged arsenic repressor fused to superfolder GFP under the control of T7 promoter
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K1758203_sequence (Version 1) |
Description
Uses T7 promotor and a strong rbs (BBa_K525998), the arsenic repressor linked with superfolding GFP (sfGFP) with a His-Tag. By linking sfGFP we want to detect bound protein via fluorescence.Notes
We clone arsR in T7-RBS-sfGFP and added a His-Tag via PCR and Gibson Assembly.Source
This is a composite part which enables a high protein expression and an easy purification of arsR fused to superfolding GFP (sfGFP). The green fluorescence of the culture is a sign for protein expression.| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0034 BBa_K525998 RBS T7 Promoter His-Tag arsR sfGFP BBa_B0010 BBa_B0012 | 21,32 1,32 24,27 1,23 1123,1146 50,402 409,1122 1155,1234 1243,1283 | feature/BioBrick engineered_region feature/BioBrick engineered_region ribosome_entry_site feature/rbs promoter feature/promoter feature/tag tag CDS feature/cds CDS feature/cds engineered_region feature/BioBrick feature/BioBrick engineered_region |