Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Team Bielefeld-CeBiTec 2015
Date created: 2015-08-28 11:00:00
Date modified: 2015-09-18 07:21:49
His-tagged blc repressor fused to superfolder GFP under the control of the T7 promoter
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1758204_sequence (Version 1) |
Description
Uses T7 promotor and a strong rbs (BBa_K525998), the blc repressor linked with superfolding GFP (sfGFP) with a His-Tag. By linking sfGFP we want to detect bound protein via fluorescence.Notes
This is a composite part which enables a high protein expression and an easy purification of blcR fused to superfolding GFP (sfGFP). The green fluorescence of the culture is a sign for protein expression.Source
We clone blcR in T7-RBS-sfGFP and added a His-Tag via PCR and Gibson Assembly.| Sequence Annotation | Location | Component / Role(s) |
| T7 promoter RBS BBa_K525998 BBa_B0034 BBa_B0012 BBa_B0010 blcR sfGFP His-Tag | 1,23 24,27 1,32 21,32 1715,1755 1627,1706 50,874 881,1594 1595,1618 | feature/promoter promoter ribosome_entry_site feature/rbs feature/BioBrick engineered_region feature/BioBrick engineered_region engineered_region feature/BioBrick engineered_region feature/BioBrick CDS feature/cds CDS feature/cds feature/tag tag |