Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Danqian Liu, Zongxiao He, Hao Jiang
Date created: 2009-09-13 11:00:00
Date modified: 2015-07-09 11:38:42
lacZalpha-ccdB-LVA coding sequence
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K176158_sequence (Version 1) |
Description
Add LVA degradation tag (widely used in C0040, C0051, C0061...) to lacZalpha-ccdB(K176003) C-terminal:[K176003 without stop codon] GCT GCA AAC GAC GAA AAC TAC GCT TTA GTA GCT [TAA TAA]
A A N D E N Y A L V A
Notes
Add LVA degradation tag (widely used in C0040, C0051, C0061...) to lacZalpha-ccdB(K176003 C-terminal:[K176003 without stop codon] GCT GCA AAC GAC GAA AAC TAC GCT TTA GTA GCT [TAA TAA]
A A N D E N Y A L V A
Source
PCR from K176003.Forward Primer: VF2(G00100)
Reverse Primer: GTTT ACTAGT A TTATTA AGC TAC TAA AGC GTA GTT TTC GTC GTT TGC AGC tattccccagaacatcaggtta
Digest with EcoRI and SpeI, ligate into pSB1A3.
| Sequence Annotation | Location | Component / Role(s) |
| lacZalpha-ccdB LVA degradation tag (AANDENYALVA) Stop a part of lacZalpha (I732006) Start 177bp and a in J32026 M13 -20 primer M13 -47 primer (K176059) M13 -40 primer g and 69bp in I732006 at in K145151 ccdB (K145151) VRccdB primer (K176058) c in K145151 | 1,474 475,507 508,513 1,165 1,3 16,16 21,37 41,64 41,57 165,165 172,173 172,474 188,215 255,255 | CDS feature/cds feature/tag tag feature/stop stop_codon engineered_region feature/BioBrick feature/start start_codon sequence_alteration feature/mutation primer_binding_site feature/primer_binding primer_binding_site feature/primer_binding primer_binding_site feature/primer_binding point_mutation feature/s_mutation feature/mutation sequence_alteration engineered_region feature/BioBrick feature/primer_binding primer_binding_site feature/s_mutation point_mutation |